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Promega p8.74 plasmid
P8.74 Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p8%2E74+plasmid/p8+74+plasmid/pmc06746825-43-31-48
Average 90 stars, based on 1 article reviews
p8.74 plasmid - by Bioz Stars, 2026-11
90/100 stars

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Transfection:

Article Title: Loss of Janus Associated Kinase 1 Alters Urothelial Cell Function and Facilitates the Development of Bladder Cancer
Article Snippet: Lentivirus stocks were prepared by transfection of 293T cells (80–90% confluence) cultured in DMEM medium and 10% heat-inactivated fetal bovine serum, with the envelope plasmid 17.5 μg pMD.G2 (VSV-G/envelop), 32.5 μg p8.74 plasmid (gag-pol) and 25 μg vector construct with the transfection reagent PEI/Opti-MEMTM following the manufacturer's instructions (Promega).

Cell Culture:

Article Title: Loss of Janus Associated Kinase 1 Alters Urothelial Cell Function and Facilitates the Development of Bladder Cancer
Article Snippet: Lentivirus stocks were prepared by transfection of 293T cells (80–90% confluence) cultured in DMEM medium and 10% heat-inactivated fetal bovine serum, with the envelope plasmid 17.5 μg pMD.G2 (VSV-G/envelop), 32.5 μg p8.74 plasmid (gag-pol) and 25 μg vector construct with the transfection reagent PEI/Opti-MEMTM following the manufacturer's instructions (Promega).

Plasmid Preparation:

Article Title: Loss of Janus Associated Kinase 1 Alters Urothelial Cell Function and Facilitates the Development of Bladder Cancer
Article Snippet: Lentivirus stocks were prepared by transfection of 293T cells (80–90% confluence) cultured in DMEM medium and 10% heat-inactivated fetal bovine serum, with the envelope plasmid 17.5 μg pMD.G2 (VSV-G/envelop), 32.5 μg p8.74 plasmid (gag-pol) and 25 μg vector construct with the transfection reagent PEI/Opti-MEMTM following the manufacturer's instructions (Promega).

Construct:

Article Title: Loss of Janus Associated Kinase 1 Alters Urothelial Cell Function and Facilitates the Development of Bladder Cancer
Article Snippet: Lentivirus stocks were prepared by transfection of 293T cells (80–90% confluence) cultured in DMEM medium and 10% heat-inactivated fetal bovine serum, with the envelope plasmid 17.5 μg pMD.G2 (VSV-G/envelop), 32.5 μg p8.74 plasmid (gag-pol) and 25 μg vector construct with the transfection reagent PEI/Opti-MEMTM following the manufacturer's instructions (Promega).



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Promega p8.74 plasmid
P8.74 Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p8%2E74+plasmid/p8+74+plasmid/pmc06746825-43-31-48
Average 90 stars, based on 1 article reviews
p8.74 plasmid - by Bioz Stars, 2026-11
90/100 stars
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